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opa1 nbp2 59770ss antibody  (Novus Biologicals)


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    Novus Biologicals opa1 nbp2 59770ss antibody
    Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and <t>OPA1</t> protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.
    Opa1 Nbp2 59770ss Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opa1+nbp2+59770ss+antibody/OPA1+Antibody+(1284B)+-+BSA+Free/pmc08275580-199-1-7
    Average 94 stars, based on 11 article reviews
    opa1 nbp2 59770ss antibody - by Bioz Stars, 2026-10
    94/100 stars

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    1) Product Images from "Mitofusion is required for MOTS‐c induced GLUT4 translocation"

    Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-93735-2

    Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and OPA1 protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.
    Figure Legend Snippet: Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and OPA1 protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.

    Techniques Used: Expressing, Modification, Western Blot, Control, Cell Analysis

    Related Articles

    Expressing:

    Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation
    Article Snippet: Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).. The OPA1 (NBP2-59770SS) antibody was obtained from Novus Biologicals (Toronto, Canada).. All the antibodies were used at a dilution of 1:1000.All the antibodies were used at a dilution of 1:1000.

    Modification:

    Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation
    Article Snippet: Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).. The OPA1 (NBP2-59770SS) antibody was obtained from Novus Biologicals (Toronto, Canada).. All the antibodies were used at a dilution of 1:1000.All the antibodies were used at a dilution of 1:1000.

    Western Blot:

    Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation
    Article Snippet: Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).. The OPA1 (NBP2-59770SS) antibody was obtained from Novus Biologicals (Toronto, Canada).. All the antibodies were used at a dilution of 1:1000.All the antibodies were used at a dilution of 1:1000.

    Control:

    Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation
    Article Snippet: Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).. The OPA1 (NBP2-59770SS) antibody was obtained from Novus Biologicals (Toronto, Canada).. All the antibodies were used at a dilution of 1:1000.All the antibodies were used at a dilution of 1:1000.

    Cell Analysis:

    Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation
    Article Snippet: Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).Primary antibodies used in immunoblotting including TFAM (ab138351), COX4 (ab202554), NRF1 (ab175932), PGC1α (ab54481), TOMM20 (ab186735), MFN2 (ab124773), GLUT4 (ab654) and GAPDH (ab8245) were purchased from Abcam (Abcam Inc, Toronto, Canada).. The OPA1 (NBP2-59770SS) antibody was obtained from Novus Biologicals (Toronto, Canada).. All the antibodies were used at a dilution of 1:1000.All the antibodies were used at a dilution of 1:1000.



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    opa1 nbp2 59770ss antibody  (Novus Biologicals)
    94
    Novus Biologicals opa1 nbp2 59770ss antibody
    Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and <t>OPA1</t> protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.
    Opa1 Nbp2 59770ss Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opa1+nbp2+59770ss+antibody/OPA1+Antibody+(1284B)+-+BSA+Free/pmc08275580-199-1-7
    Average 94 stars, based on 1 article reviews
    opa1 nbp2 59770ss antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and OPA1 protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.

    Journal: Scientific Reports

    Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation

    doi: 10.1038/s41598-021-93735-2

    Figure Lengend Snippet: Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and OPA1 protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.

    Article Snippet: The OPA1 (NBP2-59770SS) antibody was obtained from Novus Biologicals (Toronto, Canada).

    Techniques: Expressing, Modification, Western Blot, Control, Cell Analysis